Overview

  • Product name
    Anti-C7 antibody [EPR7036]
    See all C7 primary antibodies
  • Description
    Rabbit monoclonal [EPR7036] to C7
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C7 aa 800-900. The exact sequence is proprietary.
    Database link: P10643

  • Positive control
    • HepG2, Ramos, K562 and HUVEC cell lysates; Human kidney tissue; HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7036
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
    • Involvement in disease
      Defects in C7 are a cause of complement component 7 deficiency (C7D) [MIM:610102]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • Sequence similarities
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 2 Sushi (CCP/SCR) domains.
      Contains 2 TSP type-1 domains.
    • Post-translational
      modifications
      C7 has 28 disulfide bridges.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C7 antibody
      • CO7_HUMAN antibody
      • complement component 7 antibody
      • Complement component C7 antibody

    Images

    • All lanes : Anti-C7 antibody [EPR7036] (ab126786) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HUVEC cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 94 kDa
    • ab126786, at a 1/250 dilution, staining C7 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab126786, at a 1/100 dilution, staining C7 in HepG2 cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Li GS  et al. Complement components of nerve regeneration conditioned fluid influence the microenvironment of nerve regeneration. Neural Regen Res 11:682-6 (2016). WB . Read more (PubMed: 27212935) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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