Overview

  • Product name
    Anti-C7orf25 antibody
  • Description
    Rabbit polyclonal to C7orf25
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GPGERERATV LIKRINVVPD QPSERALRLV ASSKINSRSL TIFGTGDTLK AITMTANSGF VRAANNQGVK FSVFIHQPRA LTESKEALAT PLPKDYTTD, corresponding to amino acids 320-418 of Human C7orf25.

  • Positive control
    • Human urinary bladder tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121621 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121621 at 1/300 staining C7orf25 in Paraffin Embedded Human urinary bladder tissue by Immunohistochemistry.
    Note: strong nuclear positivity in urothelial cells.

References

ab121621 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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