Overview

  • Product name
    Anti-C7orf30 antibody
  • Description
    Rabbit polyclonal to C7orf30
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: AFYVVKMYKH LKCKRDPHVK IEGKDTDDWL CVDFGSMVIH LMLPETREIY ELEKLWTLRS YDDQLAQIAP ETVPEDFILG IEDDTSSVTP V, corresponding to amino acids 139-229 of Human C7orf30 (Q96EH3).

  • Positive control
    • RT4 and U-251 MG cell lysates, Human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab121631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • All lanes : Anti-C7orf30 antibody (ab121631) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
  • ab121631 at 1/250 dilution staining C7orf30 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria using ab121631.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References

ab121631 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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