Overview

  • Product nameAnti-C7orf36 antibody
    See all C7orf36 primary antibodies
  • Description
    Rabbit polyclonal to C7orf36
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LKHLKSITPP SHVVDLLDSI EDMDLCHVVP AEKKIDEAKD ERLCENNAEF NKNCSKSHSG IDCSYVECCR TQEHAHSENP SPT, corresponding to amino acids 118-200 of Human C7orf36.

  • Positive control
    • Human cerebral cortex tissue; HEK293T cells transfected with C7orf36.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-C7orf36 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and mitochondria. Recommended concentration of ab121595 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121595 at 1/50 dilution staining C7orf36 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • All lanes : Anti-C7orf36 antibody (ab121595) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

References for Anti-C7orf36 antibody (ab121595)

ab121595 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"