Anti-C8 antibody [056B-373] (ab59140)
Key features and details
- Mouse monoclonal [056B-373] to C8
- Suitable for: ELISA, IHC-Fr, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-C8 antibody [056B-373] -
Description
Mouse monoclonal [056B-373] to C8 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, IHC-Fr, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human C8. Purified human C8
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Positive control
- Kidney from post streptoccal glomerulonephritis patients.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
056B-373 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Relevance
C8 is synthesised in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 731 Human
- Entrez Gene: 732 Human
- Entrez Gene: 733 Human
- Omim: 120930 Human
- Omim: 120950 Human
- Omim: 120960 Human
- SwissProt: P07357 Human
- SwissProt: P07358 Human
see all -
Alternative names
- C8A antibody
- C8B antibody
- C8G antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab59140 has not yet been referenced specifically in any publications.