Overview

  • Product name
    Anti-C8 antibody [056B-373]
  • Description
    Mouse monoclonal [056B-373] to C8
  • Tested applications
    Suitable for: WB, ELISA, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human C8

  • Positive control
    • Kidney from post streptoccal glomerulonephritis patients.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 99% Borate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    056B-373
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 151 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    C8 is synthesised in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • C8A antibody
    • C8B antibody
    • C8G antibody
    • Complement component 8 subunit alpha antibody
    • Complement component 8 subunit beta antibody
    • complement component 8, alpha polypeptide antibody
    • complement component 8, beta polypeptide antibody
    • complement component 8, gamma polypeptide antibody
    • Complement component C8 alpha chain antibody
    • Complement component C8 beta chain antibody
    • Complement component C8 gamma chain antibody
    • MGC142186 antibody
    • MGC163447 antibody
    see all

References

ab59140 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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