Overview

  • Product name
  • Description
    Rabbit polyclonal to C8orf55
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 123-207 (LLGWDDRAFY LEARFVSLRD GFVCALLRFR QHLLGTSPER VVQHLCQRRV EPPELPADLQ HWISYNEASS QLLRMESGLS DVTKDQ) of Human C8orf55.

  • Positive control
    • Human cerebellum tissue;
      RT-4 and U-251 MG cell lysate; Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the UPF0670 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C8orf55 antibody
    • CH055_HUMAN antibody
    • DSCD75 antibody
    • Mesenchymal stem cell protein DSCD75 antibody
    • mesenchymal stem cell protein DSCD75 homolog antibody
    • THEM6 antibody
    • Thioesterase superfamily member 6 antibody
    • UPF0670 protein C8orf55 antibody
    • UPF0670 protein C8orf55 homolog antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121743 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • All lanes : Anti-C8orf55 antibody (ab121743) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab121743 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab121743.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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