Overview

  • Product nameAnti-C8orf74 antibody
    See all C8orf74 primary antibodies
  • Description
    Rabbit polyclonal to C8orf74
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: CQAVHTQMEL LQELLQRQIQ NTFAILDLKL QKKTLNLNAP TPIPPPITSH AGQEEALKPQ RASKGKKAKA RK, corresponding to amino acids 223-294 of Human C8orf74 (UniProt Q6P047).

  • Positive control
    • Human skeletal muscle tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-C8orf74 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121965 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121965, at a 1/65 dilution, staining C8orf74 in paraffin emebedded Human skeletal muscle tissue by Immunohistochemistry.

References for Anti-C8orf74 antibody (ab121965)

ab121965 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"