Overview

  • Product name
    Anti-C9 antibody [64E9]
    See all C9 primary antibodies
  • Description
    Mouse monoclonal [64E9] to C9
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human) isolated from plasma

  • Positive control
    • Human plasma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    64E9
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 63 kDa.

Target

  • Function
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities
    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 1 TSP type-1 domain.
  • Post-translational
    modifications
    Thrombin cleaves factor C9 to produce C9a and C9b.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD15 antibody
    • C9 antibody
    • C9 deficiency antibody
    • C9 deficiency with dermatomyositis antibody
    • C9D antibody
    • CO9_HUMAN antibody
    • Complement component 9 antibody
    • Complement component 9 deficiency antibody
    • Complement component C9 antibody
    • Complement component C9b antibody
    see all

Anti-C9 antibody [64E9] images

  • All lanes : Anti-C9 antibody [64E9] (ab52431) at 1/2000 dilution

    Lane 1 : Complement factor C9 isolated from human plasma
    Lane 2 : Human plasma


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-C9 antibody [64E9] (ab52431)

This product has been referenced in:
  • Ecker SM  et al. Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous. Mol Vis 17:2856-63 (2011). WB ; Human . Read more (PubMed: 22128233) »

See 1 Publication for this product

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We do not have any data on reactivity in rat. We do put all the tested species on our datasheet under "reacts with" or in the case that is it tested and proven not to work, a "does not react with" se...

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Thank you for your enquiry regardingab52431 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the event that a pr...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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