Overview

  • Product name
  • Description
    Rabbit polyclonal to C9
  • Specificity
    This product may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome. We recommend ab55811 for specifically detecting the C5b-9 complex.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human C9.

    (Peptide available as ab71329.)

  • Positive control
    • This antibody gave a positive signal in Human Placenta Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab71330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).Can be blocked with Human C9 peptide (ab71329).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities
    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 1 TSP type-1 domain.
  • Post-translational
    modifications
    Thrombin cleaves factor C9 to produce C9a and C9b.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD15 antibody
    • C9 antibody
    • C9 deficiency antibody
    • C9 deficiency with dermatomyositis antibody
    • C9D antibody
    • CO9_HUMAN antibody
    • Complement component 9 antibody
    • Complement component 9 deficiency antibody
    • Complement component C9 antibody
    • Complement component C9b antibody
    see all

Images

  • Anti-C9 antibody (ab71330) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ab71330, at a 1/2000 dilution, staining human C9 in placenta tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

References

ab71330 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your inquiry.

I can confirm that both antibodies may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome.

Both antibodieswere raised against an immu...

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Thank you for contacting us.
After discussing this matter with my colleagues in our lab, we have decided to change the target for this antibody from C5b-9 to C9. The antibody was raised against a sequence from the C9 component of the C5b-9 complex...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Specification
Placenta
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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