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Synthetic peptide derived from an internal region of Human C9.
Our Abpromise guarantee covers the use of ab92690 in the following tested applications.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 63 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml.|
IHC image of C9 staining in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92690, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab92690 has not yet been referenced specifically in any publications.