Overview

  • Product name
    Anti-C9 antibody [aE11]
    See all C9 primary antibodies
  • Description
    Mouse monoclonal [aE11] to C9
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, Flow Cyt, Functional Studiesmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Horse, Human, Pig
  • Immunogen

    Human purified MAC (membrane attack complex)

  • Positive control
    • Mucosa from patients with H. Pylori.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab90801 was filtered through a 0.2 µm filter.
  • Clonality
    Monoclonal
  • Clone number
    aE11
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/50.
IHC-Fr 1/50.
Flow Cyt 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration. aE11 inhibits platelet activation by antiphospholipid antibody serum.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • Sequence similarities
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.
    • Post-translational
      modifications
      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARMD15 antibody
      • C9 antibody
      • C9 deficiency antibody
      • C9 deficiency with dermatomyositis antibody
      • C9D antibody
      • CO9_HUMAN antibody
      • Complement component 9 antibody
      • Complement component 9 deficiency antibody
      • Complement component C9 antibody
      • Complement component C9b antibody
      see all

    Anti-C9 antibody [aE11] images

    • Detection of TCC with ab90801 diluted 20x in PBSA.

    References for Anti-C9 antibody [aE11] (ab90801)

    This product has been referenced in:
    • Meuwissen M  et al. Colocalisation of intraplaque C reactive protein, complement, oxidised low density lipoprotein, and macrophages in stable and unstable angina and acute myocardial infarction. J Clin Pathol 59:196-201 (2006). IHC-Fr ; Human . Read more (PubMed: 16443738) »
    • Berstad AE  et al. Epithelium related deposition of activated complement in Helicobacter pylori associated gastritis. Gut 40:196-203 (1997). IHC-P, IF ; Human . Read more (PubMed: 9071931) »

    See all 5 Publications for this product

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    There are currently no Abreviews or Questions for ab90801.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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