Overview

  • Product nameAnti-C9orf135 antibody
    See all C9orf135 primary antibodies
  • Description
    Rabbit polyclonal to C9orf135
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 1-71 (MDSLDRSCQD WCDRKQHWLE IGPPDLVERK GSLTLRSHHK KYSKPVLVYS WHRDREAFPK GYDIEGPEKV K) of Human C9orf135.

  • Positive control
    • Human stomach tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-C9orf135 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli, nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121659 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-C9orf135 antibody (ab121659)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing C9orf135 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab121659, at 1/500 dilution, staining C9orf135 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

References for Anti-C9orf135 antibody (ab121659)

ab121659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"