Overview

  • Product name
  • Description
    Rabbit polyclonal to C9orf140
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ELEQEKEVLL QGLEMMARGR DWYQQQLQRV QERQRRLGQS RASADFGAAG SPRPLGRLLP KVQEVARC, corresponding to internal sequence amino acids 234-301 of Human C9orf140.

  • Positive control
    • Human rectum tissue; U 2 OS cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • Tissue specificity
    Expressed in 5-month-old fetal tissues, including stomach, intestine, colon, liver, brain, lung, heart, spleen and kidney. Undetectable in non-cancerous adult tissues. Expressed in many primary gastric carcinoma, but almost not in adjacent normal mucosa. Expressed preferentially in M and G1 phases, compared to S and G2 phases.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010317E24Rik antibody
    • C9orf140 antibody
    • Chromosome 9 open reading frame 140 antibody
    • CI140_HUMAN antibody
    • p42.3 antibody
    • Protein C9orf140 antibody
    • SAPCD2 antibody
    • Suppressor APC domain containing 2 antibody
    • Suppressor APC domain containing protein 2 antibody
    • TS/MDEP antibody
    • Tumor specificity and mitosis phase-dependent expression protein antibody
    see all

Images

  • ab126342, at 1/1000 dilution, staining C9orf140 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • ab126342, at 4µg/ml, staining C9orf140 in in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References

ab126342 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot

Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
14000 cells
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 4-12% Bis-Tris Gel,1.0 mm)
Sample
Human Cell lysate - whole cell (retinal pigment epithelium (RPE) 1 cells)
Specification
retinal pigment epithelium (RPE) 1 cells
Treatment
different siRNAs for 72h
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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