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Rabbit polyclonal to C9orf171
antigen sequence: SSAVQKVIPS LAGHHIKGGP QAELGKPRER SYSLPGINFN YGLYIRGLDG GVPEAIGRWN VFKQQPTCPH ELTRNYIAMN, corresponding to amino acids 81-160 of Human C9orf171.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- C9orf171 antibody
- Chromosome 9 open reading frame 171 antibody
- CI171_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-C9orf171 antibody (ab121835)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab121835 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf171 antibody (ab121835)
ab121835 at 1/75 dilution staining C9orf171 in Paraffin-embedded Human Testis tissue by Immunohistochemistry. Note: strong positivity in sertoli cells.
has not yet been referenced specifically in any publications.
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