Overview

  • Product name
  • Description
    Rabbit polyclonal to C9orf172
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey, Gorilla
  • Immunogen

    amino acids 307-400 of Human C9orf172.

  • Positive control
    • Hman bone marrow tissue; RT4, U251 MG cell lysates, Human liver tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and focal adhesions. Recommended concentration of ab121654 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-C9orf172 antibody (ab121654) at 1/500 dilution

    Lane 1 : RT4 cell lysates
    Lane 2 : U251 MG cell lysates
    Lane 3 : Human Plasma

    Lane 4 : Liver tissue lysate
    Lane 5 : Tonsil tissue lysate
  • ab121654, at 1/150 dilution, staining C9orf172 in Human bone marrow tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References

ab121654 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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