Customer reviews and FAQs

Overview

  • Product name
    Anti-C9orf5 antibody
  • Description
    Rabbit polyclonal to C9orf5
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 268-350 (AELPGQVISM AASTLANLAI SITGYESSSE DQPSTQPAEA VDRGESAPTL STSPSPSSPS PTSPSPTLGR RRPEIGTFLR KKK) of Human C9orf5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human stomach tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-C9orf5 antibody images

  • Immunocytochemistry/ Immunofluorescence - Anti-C9orf5 antibody (ab121781)
    Immunocytochemistry/ Immunofluorescence - Anti-C9orf5 antibody (ab121781)
    Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121781 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf5 antibody (ab121781)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf5 antibody (ab121781)
    ab121781, at 1/1500 dilution, staining C9orf5 in paraffin-embedded Human stomach tissue, by Immunohistochemistry.

References for Anti-C9orf5 antibody (ab121781)

ab121781 has not yet been referenced specifically in any publications.

Publishing research using ab121781? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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