Overview

  • Product nameAnti-C9orf66 antibody
    See all C9orf66 primary antibodies
  • Description
    Rabbit polyclonal to C9orf66
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence RPTRLPRRLS PFWDPATCKN LEGGAGEVVR GRDPRRLRTS RSTEILGEDL AGPSAGAAAR PAAPPPQPRE PGA, corresponding to N terminal amino acids 7-79 of Human C9orf66 (UniProt ID: Q5T8R8).

  • Positive control
    • Human breast tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-C9orf66 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and focal adhesions. Recommended concentration of ab122288 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122288, at a 1/175 dilution, staining C9orf66 in paraffin embedded Human breast tissue by Immunohistochemistry.

References for Anti-C9orf66 antibody (ab122288)

ab122288 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"