Overview

  • Product name
    Anti-C9orf80 antibody
  • Description
    Rabbit polyclonal to C9orf80
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: KEKRKLLMQN QSSTNHPGAS IALSRPSLNK DFRDHAEQQH IAAQQKAALQ HAHAHSSGYF ITQDSAFGNL, corresponding to amino acids 24-93 of Human C9orf80.

  • Positive control
    • Human adrenal gland tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells; Human cell line U 2 OS.

Applications

Our Abpromise guarantee covers the use of ab121623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Function
    Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.
  • Sequence similarities
    Belongs to the SOSS-C family.
  • Cellular localization
    Nucleus. Localizes to nuclear foci following DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromosome 9 open reading frame 80 antibody
    • hSSBIP1 antibody
    • MISE antibody
    • RP11-276E15.2 antibody
    • Sensor of single-strand DNA complex subunit C antibody
    • Sensor of ssDNA subunit C antibody
    • Single-stranded DNA-binding protein-interacting protein 1 antibody
    • SOSS C antibody
    • SOSS complex subunit C antibody
    • SOSS-C antibody
    • SOSSC antibody
    • SOSSC_HUMAN antibody
    • SSB-interacting protein 1 antibody
    • Ssbip1 antibody
    see all

Images

  • ab121623 at 1/40 dilution staining C9orf80 in Paraffin Embedded Human adrenal gland tissue by Immunohistochemistry.
    Note: strong nuclear positivity in cortical cells.
  • All lanes : Anti-C9orf80 antibody (ab121623) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • ab121623 at 4 µg/ml staining C9orf80 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
    Note: positivity in nuclei but not nucleoli.

References

ab121623 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121623.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up