Overview

  • Product nameAnti-C9ORF91 antibody
  • Description
    Rabbit polyclonal to C9ORF91
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 192-305 of Human C9ORF91.

  • Positive control
    • C9ORF91 over-expression HEK293T lysate; Human prostate tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-C9ORF91 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121527 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121527 at 1/500 dilution staining C9ORF91 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • All lanes : Anti-C9ORF91 antibody (ab121527) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T cell lysate
    Lane 2 : C9ORF91 over-expression HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Developed using the ECL technique

References for Anti-C9ORF91 antibody (ab121527)

ab121527 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"