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- Secondary antibodies
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Product name
Anti-cIAP1 antibody
See all cIAP1 products (4) ...
Description
Rabbit polyclonal to cIAP1
Specificity
This antibody detects both c-IAP1 and c-IAP2.
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1
Positive control
Human lung cell lysate can be used as positive control.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa.
IHC-P: Use a concentration of 10 µg/ml.
Target
Function
Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
Tissue specificity
Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ13490
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- API 1 antibody
- API1 antibody
- Apoptosis inhibitor 1 antibody
see all
Database links
- Entrez Gene: 329 Human
- Entrez Gene: 11797 Mouse
- Omim: 601712 Human
- SwissProt: Q13490 Human
- SwissProt: Q62210 Mouse
- Unigene: 696238 Human
- Unigene: 335659 Mouse
Anti-cIAP1 antibody images:
Western blot - cIAP1 antibody (ab25939)
Lane 1 : Anti-cIAP1 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-cIAP1 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-cIAP1 antibody (ab25939) at 4 µg/ml
Lane 1 : Human lung lysate
Lane 2 : Human lung lysate
Lane 3 : Human lung lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - cIAP1 antibody (ab25939)
ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC
Immunocytochemistry/ Immunofluorescence-cIAP1 antibody(ab25939)
ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-cIAP1 antibody (ab25939)
This product has been referenced in:
- Gualco E et al. IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors. Cell Death Differ 17:439-51 (2010). IHC-P; Mouse.Read more (PubMed: 19834489) »
- Zhao TT et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ : (2008). WB; Human.Read more (PubMed: 18846111) »
See all 2 publications for this product
Publishing research using ab25939? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"