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ab25892 |
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ab23423 has been referenced in 3 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-cIAP2 antibody (ab23423) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 71 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa (possible cleavage fragment,cross reactivity),49 kDa (possible cleavage fragment,cross reactivity).
ICC/IF image of ab23423 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23423, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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