Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-cIAP2 antibody
See all cIAP2 products (6) ...
Description
Rabbit polyclonal to cIAP2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human cIAP2.
(Peptide available as ab25892.)
Positive control
This antibody gave a positive signal in HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 71 kDa (predicted molecular weight: 68 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
Tissue specificity
Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
Involvement in disease
Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ13489
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CIAP 2 antibody
- Inhibitor of apoptosis protein 1 antibody
- AIP 1 antibody
see all
Database links
- Entrez Gene: 330 Human
- Entrez Gene: 11796 Mouse
- Entrez Gene: 78971 Rat
- Omim: 601721 Human
- SwissProt: Q13489 Human
- SwissProt: O08863 Mouse
- Unigene: 127799 Human
- Unigene: 2026 Mouse
see all
Anti-cIAP2 antibody images:
Western blot - cIAP2 antibody (ab23423)
Anti-cIAP2 antibody (ab23423) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 71 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa (possible cleavage fragment,cross reactivity),49 kDa (possible cleavage fragment,cross reactivity).
Immunocytochemistry/ Immunofluorescence - cIAP2 antibody (ab23423)
ICC/IF image of ab23423 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23423, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
References for Anti-cIAP2 antibody (ab23423)
This product has been referenced in:
- Huang Yet al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell 22:1389-97 (2011). WB; Human.Read more (PubMed: 21307340) »
- Wang Zet al. Inhibition of Hepatitis B Virus Replication by cIAP2 Involves Accelerating the Ubiquitin-Proteasome-Mediated Destruction of Polymerase. J Virol 85:11457-67 (2011).Read more (PubMed: 21865390) »
See all 3 publications for this product
Publishing research using ab23423? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"