cIAP2 antibody (ab9705)

Product Name 

cIAP2 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to cIAP2

Immunogen 

Synthetic peptide: CRSTIKGTVRT, corresponding to C terminal amino acids 591-601 of Human cIAP2.
BLAST 'CRSTIKGTVRT' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Western Blot – Approx 80 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 µg/ml.

Please note that we get a slightly higher MW band compared to the predicted size of 66kDa according to NP_001156 . We did not find anything in the literature to suggest otherwise. Peptide neutralisation experiments indicate that the 80kDa band is completely blocked when primary antibody is incubated with the blocking peptide. Moreover, BIRC3 has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Positive control 
(see definition)

Jurkat cell lysate.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs

Relevance 

cIAP2 is an apoptotic suppressor. Apoptosis is triggered by a variety of stimuli including members of the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of cIAP (cIAP1 and cIAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The cIAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF alpha by its receptor. Omi, a negative regulator of cIAP, inhibits its activity by catalytically cleaving cIAP. Another negative regulator, Smac/DIABLO, acts by enhancing the autoubiquitization activity of cIAP.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

GenBank Accession Number – NP_001156
LocusLink ID - 330
Official Gene Symbol - BIRC3

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References for cIAP2 antibody (ab9705)

  This product has been used in:

Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). PubMed: 18413714

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