Overview

  • Product name
  • Description
    Rabbit polyclonal to CA150
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human transcription elongation regulator 1 (CA150) encoded within exon 1 (LocusLink ID 10915).

  • Positive control
    • Nuclear extract from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.
  • Tissue specificity
    Detected in brain neurons.
  • Sequence similarities
    Contains 6 FF domains.
    Contains 3 WW domains.
  • Domain
    The FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE](2-5)-[FWY]-[DE](2-5).
    The WW domains bind Pro-rich domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 150 antibody
    • Co activator of 150 kDa antibody
    • Coactivator of 150 kDa antibody
    • MGC133200 antibody
    • TAF 2S antibody
    • TAF2S antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II S 150kD antibody
    • TATA box binding protein associated factor 2S antibody
    • TATA box binding protein associated factor RNA polymerase II S 150kD antibody
    • TATA box-binding protein-associated factor 2S antibody
    • TBP-associated factor, RNA polymerase II, 150-kD antibody
    • TCERG 1 antibody
    • TCERG1 antibody
    • TCRG1_HUMAN antibody
    • Transcription elongation regulator 1 antibody
    • Transcription factor CA 150 antibody
    • Transcription factor CA150 antibody
    • Urn1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling CA150 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 150 kDa

    ab10481 detection of human CA150 by Western blot. Sample: Nuclear extract (5 µg) from HeLa cells. Antibody: ab10481 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min.


    ab10481 detection of human CA150 by Western blot. Sample: Nuclear extract (5 µg) from HeLa cells. Antibody: ab10481 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NB

References

ab10481 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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