Overview

  • Product name
    Anti-CA150 antibody [TCEAD79A]
    See all CA150 primary antibodies
  • Description
    Mouse monoclonal [TCEAD79A] to CA150
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa cells; HeLa and NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab50812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/50. Predicted molecular weight: 124 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.
  • Tissue specificity
    Detected in brain neurons.
  • Sequence similarities
    Contains 6 FF domains.
    Contains 3 WW domains.
  • Domain
    The FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE](2-5)-[FWY]-[DE](2-5).
    The WW domains bind Pro-rich domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 150 antibody
    • Co activator of 150 kDa antibody
    • Coactivator of 150 kDa antibody
    • MGC133200 antibody
    • TAF 2S antibody
    • TAF2S antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II S 150kD antibody
    • TATA box binding protein associated factor 2S antibody
    • TATA box binding protein associated factor RNA polymerase II S 150kD antibody
    • TATA box-binding protein-associated factor 2S antibody
    • TBP-associated factor, RNA polymerase II, 150-kD antibody
    • TCERG 1 antibody
    • TCERG1 antibody
    • TCRG1_HUMAN antibody
    • Transcription elongation regulator 1 antibody
    • Transcription factor CA 150 antibody
    • Transcription factor CA150 antibody
    • Urn1 antibody
    see all

Images

  • Anti-CA150 antibody [TCEAD79A] (ab50812) at 1/50 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 124 kDa
  • ab50812 at 1/10 dilution staining human TCERG1 in HeLa cells by Immunocytochemistry. Secondary antibody: 1/200 AlexaFluor®488 Goat Anti-mouse IgG.
  • Anti-CA150 antibody [TCEAD79A] (ab50812) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 124 kDa

References

ab50812 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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