Overview

  • Product nameAnti-CA19-9 antibody [106-3A3]
    See all CA19-9 primary antibodies
  • Description
    Mouse monoclonal [106-3A3] to CA19-9
  • Specificity Does not cross-react with CA125, CA72-4, CA242, PSA and CEA.
  • Tested applicationsSuitable for: Indirect ELISA, Sandwich ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human colorectal adenocarcinoma cell-line COLO205.

Properties

Applications

Our Abpromise guarantee covers the use of ab115686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Alternative names
    • CA19.9 antibody
    • Ovarian tumor antigen antibody
    • Pancreatic tumor antigen antibody
    • Sialyl Lewis a antibody

Anti-CA19-9 antibody [106-3A3] images

  • Overlay histogram showing PANC1 cells stained with ab115686 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115686, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Titration curve of ab115686 in indirect ELISA Antigen: CA19-9 Antigen coated at 30 Units per well. Antibody: Dilution series of ab115686 followed by Goat anti-Mouse IgG Fc:HRP conjugate and TMB substrate.

References for Anti-CA19-9 antibody [106-3A3] (ab115686)

ab115686 has not yet been referenced specifically in any publications.

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