Overlay histogram showing PANC1 cells stained with ab115686 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115686, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Titration curve of ab115686 in indirect ELISA
Antigen: CA19-9 Antigen coated at 30 Units per well.
Antibody: Dilution series of ab115686 followed by Goat anti-Mouse IgG Fc:HRP conjugate and TMB substrate.
References for Anti-CA19-9 antibody [106-3A3] (ab115686)
has not yet been referenced specifically in any publications.
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