Overview

  • Product nameAnti-CA19-9 antibody [121SLE]
    See all CA19-9 primary antibodies
  • Description
    Mouse monoclonal [121SLE] to CA19-9
  • SpecificityThe antibody recognizes Sialyl Lewis a containing glycolipids.
  • Tested applicationsSuitable for: Flow Cyt, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in human breast and human kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab3982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 22490524
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

Target

  • Alternative names
    • CA19.9 antibody
    • Ovarian tumor antigen antibody
    • Pancreatic tumor antigen antibody
    • Sialyl Lewis a antibody

Anti-CA19-9 antibody [121SLE] images

  • ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).

  • All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml

    Lane 2 : Human breast tissue lysate - total protein (ab30090)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-CA19-9 antibody [121SLE] (ab3982)

This product has been referenced in:
  • Nordén R  et al. Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors. Glycobiology 23:310-21 (2013). Flow Cyt ; Human . Read more (PubMed: 23144050) »
  • Gebauer F  et al. Selectin binding is essential for peritoneal carcinomatosis in a xenograft model of human pancreatic adenocarcinoma in pfp--/rag2-- mice. Gut : (2012). Flow Cyt ; Human . Read more (PubMed: 22490524) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Melanoma cell line)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Melanoma cell line
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"