ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).
Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)
All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml
Lane 2 : Human breast tissue lysate - total protein (ab30090) Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 20 µg per lane.
Secondary Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 32 kDa Observed band size : 32 kDa Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
References for Anti-CA19-9 antibody [121SLE] (ab3982)
This product has been referenced in:
Nordén R et al. Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors. Glycobiology23:310-21 (2013).
Read more (PubMed: 23144050) »
Gebauer F et al. Selectin binding is essential for peritoneal carcinomatosis in a xenograft model of human pancreatic adenocarcinoma in pfp--/rag2-- mice. Gut : (2012).
Read more (PubMed: 22490524) »