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Mouse monoclonal [121SLE] to CA19-9
The antibody recognizes Sialyl Lewis a containing glycolipids.
Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.
In Western Blot, this antibody gave a positive signal in human breast and human kidney tissue lysates.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody
ab116024 as a replacement.
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
>95% by SDS-PAGE
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22490524
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
Ovarian tumor antigen antibody
Pancreatic tumor antigen antibody
Sialyl Lewis a antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)
ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).
Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)
All lanes :
Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml
Lane 2 :
Human breast tissue lysate - total protein (
Lane 3 :
Human kidney tissue lysate - total protein (
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
50 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"