Anti-CA50 antibody [93] (ab62865)

Overview

  • Product nameAnti-CA50 antibody [93]
  • Description
    Mouse monoclonal [93] to CA50
  • Tested applicationsSuitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human CA50

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number93
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use a concentration of 0.0002 µg/ml.

Target

  • RelevanceCA50 is a carcinoma associated carbohydrate antigen
  • Alternative names
    • Antigen CA50 antibody
    • CA 50 antibody
    • CA 50 antigen antibody
    • CA50 antigen antibody
    • Carbohydrate antigen CA50 antibody
    • Tumour marker CA50 antibody
    see all

Anti-CA50 antibody [93] images

  • ICC/IF image of ab62865 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62865, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CA50 antibody [93] (ab62865)

ab62865 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Unfortunately, we do not have any other antibodies against CA50 that you could use in an ELISA assay. You could try searching http://www.biocompare.com/ and see if you could locate another antibody and antigen using t...

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Thank you for your reply.

Which antibodies is it that you are interested in? The only CA 50 antibody that we have in the catalogue is ab62865, which you already have,

If there is another target that you have in mind, you could search ...

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Thank you for your reply.

For some our primary antibody targets, we also have a corresponding kit that you can purchase, which includes all of the necessary reagents for performing an ELISA. For CA50, we do not have any such kits, but you can ...

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Thank you for your reply.

I have talked to the lab and below is the protocol that was used to test ab62865 in ELISA:

1. Coat ELISA plate overnight with CA50 at 1mg/ml in pH=9.5 carbonate buffer.

2. Block with 3% gelatin PBS f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"