Anti-CABP antibody (ab93853)

Overview

  • Product name
  • Description
    Rabbit polyclonal to CABP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CABP.

    (Peptide available as ab108465.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse cerebellum; Mouse hippocampus; Mouse spinal cord.

Properties

Applications

Our Abpromise guarantee covers the use of ab93853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D (By similarity). Inhibits TRPC5 channels. Prevents NMDA receptor-induced cellular degeneration.
  • Tissue specificity
    Retina and brain. Somatodendritic compartment of neurons. Calbrain was found exclusively in brain where it is abundant in the hippocampus, habenular area in the epithalamus and in the cerebellum.
  • Sequence similarities
    Contains 4 EF-hand domains.
  • Domain
    EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.
  • Post-translational
    modifications
    Phosphorylated. The phosphorylation regulates the activity.
  • Cellular localization
    Cytoplasm > cell cortex. Cell membrane. S-CaBP1 is localized at or near the plasma membrane; Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cell membrane. Golgi apparatus. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane and Cytoplasm > cytoskeleton. L-CaBP1 is associated most likely with the cytoskeletal structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • CABP 1 antibody
    • CaBP1 antibody
    • CABP1_HUMAN antibody
    • Calbrain antibody
    • Calcium binding protein 1 antibody
    • Calcium binding protein 5 antibody
    • Calcium-binding protein 1 antibody
    • Caldendrin antibody
    • HCALB BR antibody
    • HCALB_BR antibody
    see all

Anti-CABP antibody images

  • All lanes : Anti-CABP antibody (ab93853) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 85 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-CABP antibody (ab93853)

ab93853 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (primary hippocampal neurons)
Specification
primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - ice-cold methanol (optional)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hippocampal neurons)
Loading amount
20 µg
Specification
primary hippocampal neurons
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 14 2012

Vielen Dank für Ihre Anfrage.

Der Anti-CABP-Antikörper ab93853wurde nach einem Standard-Westernblot-Protokoll getestet, das auch auf unserer Webseite zu finden ist(siehe Anhang und http://www.abcam.com/index.html?pageconfig=popular...

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