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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat CACNA1A.
(Peptide available as ab32641.)
Our Abpromise guarantee covers the use of ab32642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 190 kDa (predicted molecular weight: 268 kDa).Can be blocked with Rat CACNA1A peptide (ab32641). PubMed: 21293071
CACNA1a has a major form with an apparent molecular mass of 190 kDa and a minor, full-length form with an apparent molecular mass of 220 kDa. [PMID:7673157]
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32642 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.