Overview

  • Product nameAnti-CACNA1C antibody
    See all CACNA1C primary antibodies
  • Description
    Rabbit polyclonal to CACNA1C
  • Tested applicationsSuitable for: WB, IP, ICC, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog
    Predicted to work with: Guinea pig
  • Immunogen

    Synthetic peptide corresponding to Rat CACNA1C aa 818-835 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    C-TTKINMDDLQPSENEDKS

  • Positive control
    • Rat brain or heart
  • General notesFurther dilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. This antibody is supplied with 40ug control peptide (immunogen).

Properties

Applications

Our Abpromise guarantee covers the use of ab58552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 249 kDa.
Note: The alpha-subunit is highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include a protease inhibitor cocktail. When using as a negative control, preincubate 1ug of the control peptide with 1ug of antibody for 1hr at room temperature.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
  • Tissue specificityExpressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
  • Involvement in diseaseTimothy syndrome
    Brugada syndrome 3
  • Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
  • DomainEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
  • Post-translational
    modifications
    Phosphorylation by PKA activates the channel.
  • Cellular localizationMembrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha-1 polypeptide antibody
    • cardiac muscle antibody
    • isoform 1 antibody
    • L type antibody
    • CAC1C_HUMAN antibody
    • CACH 2 antibody
    • CACH2 antibody
    • CACN 2 antibody
    • CACN2 antibody
    • CACNA1C antibody
    • CACNL1A1 antibody
    • Calcium channel antibody
    • Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
    • Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
    • Calcium channel voltage dependent L type alpha 1C subunit antibody
    • CaV1.2 antibody
    • CCHL1A1 antibody
    • DHPR alpha 1 antibody
    • DHPR alpha 1 subunit antibody
    • LQT8 antibody
    • TS antibody
    • Voltage dependent L type calcium channel alpha 1C subunit antibody
    • Voltage dependent L type calcium channel subunit alpha 1C antibody
    • Voltage gated calcium channel alpha subunit Cav1.2 antibody
    • Voltage gated calcium channel subunit alpha Cav1.2 antibody
    • Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1C antibody
    • Voltage-gated calcium channel subunit alpha Cav1.2 antibody
    see all

References for Anti-CACNA1C antibody (ab58552)

This product has been referenced in:
  • Smith LL  et al. Bridging integrator 1 (Bin1) deficiency in zebrafish results in centronuclear myopathy. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24549043) »
  • Besser J  et al. MiRNA-1/133a clusters regulate adrenergic control of cardiac repolarization. PLoS One 9:e113449 (2014). WB ; Mouse . Read more (PubMed: 25415383) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (muscle)
Specification muscle
Fixative Acetone
Permeabilization No
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Verified customer

Submitted May 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"