Overview

  • Product nameAnti-CACNA1C antibody [S57-46]
    See all CACNA1C primary antibodies
  • Description
    Mouse monoclonal [S57-46] to CACNA1C
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog, Human
  • Immunogen

    Fusion protein corresponding to Rabbit CACNA1C aa 1507-1733 (C terminal).

  • Positive control
    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF).
IHC-Fr Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF).
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa. If results are poor, use lysate without boiling, heat at 37°C for 15 minutes.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
  • Tissue specificityExpressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
  • Involvement in diseaseTimothy syndrome
    Brugada syndrome 3
  • Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
  • DomainEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
  • Post-translational
    modifications
    Phosphorylation by PKA activates the channel.
  • Cellular localizationMembrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha-1 polypeptide antibody
    • cardiac muscle antibody
    • isoform 1 antibody
    • L type antibody
    • CAC1C_HUMAN antibody
    • CACH 2 antibody
    • CACH2 antibody
    • CACN 2 antibody
    • CACN2 antibody
    • CACNA1C antibody
    • CACNL1A1 antibody
    • Calcium channel antibody
    • Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
    • Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
    • Calcium channel voltage dependent L type alpha 1C subunit antibody
    • CaV1.2 antibody
    • CCHL1A1 antibody
    • DHPR alpha 1 antibody
    • DHPR alpha 1 subunit antibody
    • LQT8 antibody
    • TS antibody
    • Voltage dependent L type calcium channel alpha 1C subunit antibody
    • Voltage dependent L type calcium channel subunit alpha 1C antibody
    • Voltage gated calcium channel alpha subunit Cav1.2 antibody
    • Voltage gated calcium channel subunit alpha Cav1.2 antibody
    • Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1C antibody
    • Voltage-gated calcium channel subunit alpha Cav1.2 antibody
    see all

Anti-CACNA1C antibody [S57-46] images

  • All lanes : Anti-CACNA1C antibody [S57-46] (ab84814) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from DHPR alpha 1 transfected CHO cells
  • ab84814 staining CACNA1C in human hippocampus tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin embedded tissue sections). Tissue underwent heat mediate fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A Fluorophore conjugated goat anti-mouse was used as secondary at 1/50 dilution.

  • ab84814 staining CACNA1C in mouse brain tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue section. Tissue underwent heat mediated fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A HRP conjugated secondary was used at 10 dilution.

  • ab84814 staining CACNA1C in rabbit heart tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with paraformaldehyde, permeabilized using Triton X-100 and then incubated with ab84814 at a 1/100 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 555 conjugated goat anti-mouse monoclonal, used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab84814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84814, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-CACNA1C antibody [S57-46] (ab84814)

This product has been referenced in:
  • Guarnieri S  et al. New insights into the relationship between mIGF-1-induced hypertrophy and Ca2+ handling in differentiated satellite cells. PLoS One 9:e107753 (2014). WB ; Mouse . Read more (PubMed: 25229238) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Horse Tissue sections (Skeletal muscles)
Specification Skeletal muscles
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (Skeletal muscles)
Specification Skeletal muscles
Fixative Acetone/methanol 1:1
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

DISCOUNT CODE: ***
Expiration date: October 29th, 2012

I am very pleased to hear you would like to accept our offer and test ab84814 in dog. This code will give you: 1 free primary antibody before the expiration date. To redeem this offe...

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According to our records, ab84814 was proving difficult to use in IHC-Fr and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better unders...

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Thank you for submitting an Abreview of ab84814. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

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Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (atrium)
Specification atrium
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Thank you for your reply and for completing of customer survey follow up email.
I am sorry that the replacement antibody that we sent you, ab81980 did not prove to be successful for you.
Therefore I have processed your request to receive a re...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 17 2012

Thank you for contacting Abcam.
I have processed your request to receive ab81980 as a replacement for ab84814 and your new order number is ********.
I hope that this new antibody is successful and if there is anything else I can help you with...

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Thank you for reply.
Out of either ab81980 or ab8109, I would recommend ab81980, as we have a western blot image for that one on our website. We do not have any more data for either antibody, although both are guaranteed to work in western blot on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"