Anti-CACNA1H antibody [N55/10] (ab84815)
Key features and details
- Mouse monoclonal [N55/10] to CACNA1H
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CACNA1H antibody [N55/10]
See all CACNA1H primary antibodies -
Description
Mouse monoclonal [N55/10] to CACNA1H -
Host species
Mouse -
Specificity
No cross-reactivity against Cav1.3 and Cav3.1
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Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Human CACNA1H aa 1000-1300.
Database link: O95180 -
Positive control
- IHC-P: Human hippocampus tissue. IHC-Fr: Frozen human brain tissue.
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General notes
The clone number has been updated from S55-10 to N55/10, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N55/10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000.
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WB |
1/1000. Predicted molecular weight: 259 kDa.
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IHC-Fr |
1/1000.
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Notes |
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IHC-P
1/1000. |
WB
1/1000. Predicted molecular weight: 259 kDa. |
IHC-Fr
1/1000. |
Target
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Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. -
Tissue specificity
Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific. -
Involvement in disease
Defects in CACNA1H are a cause of susceptibility to idiopathic generalized epilepsy type 6 (IGE6) [MIM:611942]. IGE is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. IGE6 is a polygenic and multifactorial disease. -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily. -
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsIn response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8912 Human
- Entrez Gene: 58226 Mouse
- Entrez Gene: 114862 Rat
- Omim: 607904 Human
- SwissProt: O95180 Human
- SwissProt: O88427 Mouse
- SwissProt: Q9EQ60 Rat
- Unigene: 459642 Human
see all -
Alternative names
- Alpha1 3.2 antibody
- Alpha13.2 antibody
- CAC1H_HUMAN antibody
see all
Images
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Immunohistochemical analysis of frozen Human brain tissue sections staining CACNA1H. Samples were fixed with 10% Formalin solution and incubated with primary antibody at 1:1000 dilution for 1 hour at room temperature. A biotinylated goat anti-mouse was used as the secondary antibody. Mayer Hematoxylin was used as a nuclear stain.
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Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of human hippocampus tissue labelling CACNA1H with ab84815 at 1:1000 dilution, followed by secondary antibody Goat anti-mouse (green) at 1:50 for 1 hour at RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab84815 has been referenced in 2 publications.
- Kürtüncü M et al. CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL). Cephalalgia : (2012). WB ; Human . PubMed: 23111027
- Ohkubo T & Yamazaki J T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells. Int J Oncol 41:267-75 (2012). PubMed: 22469755