Overview

  • Product name
    Anti-CACNB1 antibody [N7/18]
    See all CACNB1 primary antibodies
  • Description
    Mouse monoclonal [N7/18] to CACNB1
  • Specificity
    No cross reactivity against CACNB4.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Synthetic peptide:

    PMEVFDPSPQGKYSKR

    , corresponding to amino acids 19-34 of Rat CACNB1 (NP_059042).

  • Positive control
    • Rat brain lysate.
  • General notes

    The clone number has been updated from S7-18 to N7/18, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab85020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 66 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
IHC-P Use a concentration of 0.1 - 1 µg/ml.

Target

  • Function
    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
  • Tissue specificity
    Isoform 1 and isoform 3 are expressed in brain, heart, spleen, central nervous system and neuroblastoma cells. Isoform 2 is expressed in skeletal muscle.
  • Sequence similarities
    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.
  • Cellular localization
    Cell membrane > sarcolemma.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAB1 antibody
    • CACB1_HUMAN antibody
    • CACNB1 antibody
    • CACNLB1 antibody
    • Calcium channel L type beta 1 polypeptide antibody
    • Calcium channel voltage dependent beta 1 subunit antibody
    • Calcium channel voltage-dependent subunit beta 1 antibody
    • CCHLB1 antibody
    • Dihydropyridine sensitive L type calcium channel beta 1 subunit antibody
    • MGC41896 antibody
    • Voltage dependent L type calcium channel beta 1 subunit antibody
    • Voltage-dependent L-type calcium channel subunit beta-1 antibody
    see all

Images

  • ab85020 staining CACNB1 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Hippocampal synaptosome preparation)
Loading amount
5 µg
Specification
Hippocampal synaptosome preparation
Gel Running Conditions
Reduced Denaturing (Bis Tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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