Overview

  • Product name
  • Description
    Mouse monoclonal to CACNB2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PSSRKSTPPS SAIDIDATGL DAEENDIPAN HRSPKPSANS VTSPHSKEKR MPFFKKTEHT PPYDVVPSMR PVVLVGPSLK GYEVTDMMQ, corresponding to amino acids 213-302 of Human CACNB2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 74 kDa.
ELISA 1/500. Has not been tested in ELISA on endogenous samples.

Target

  • Function
    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
  • Tissue specificity
    Expressed in all tissues.
  • Involvement in disease
    Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRS4) [MIM:611876]. BRS4 is a heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.
  • Sequence similarities
    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.
  • Cellular localization
    Cell membrane > sarcolemma.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW060387 antibody
    • Ca(V) beta 2 antibody
    • CAB2 antibody
    • CACB2_HUMAN antibody
    • CACNB2 antibody
    • CACNLB2 antibody
    • Calcium channel voltage dependent subunit beta 2 antibody
    • Calcium channel voltage-dependent subunit beta 2 antibody
    • Calcium channel, voltage dependent, beta 2 subunit antibody
    • CAVB2 antibody
    • Cavbeta2 antibody
    • Cchb2 antibody
    • FLJ23743 antibody
    • Lambert Eaton Myasthenic syndrome antigen antibody
    • Lambert Eaton myasthenic syndrome antigen B antibody
    • Lambert-Eaton myasthenic syndrome antigen B antibody
    • MGC129334 antibody
    • MGC129335 antibody
    • Myasthenic (Lambert Eaton) syndrome antigen B antibody
    • Myasthenic syndrome antigen B antibody
    • MYSB antibody
    • Voltage dependent L type calcium channel subunit beta 2 antibody
    • Voltage-dependent L-type calcium channel subunit beta-2 antibody
    see all

Anti-CACNB2 antibody images



  • Predicted band size : 74 kDa
    CACNB2 antibody (ab54920) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

References for Anti-CACNB2 antibody (ab54920)

This product has been referenced in:
  • Rougier JS  et al. Ubiquitin-specific protease USP2-45 acts as a molecular switch to promote a2d-1-induced downregulation of Ca v1.2 channels. Pflugers Arch N/A:N/A (2014). Read more (PubMed: 25366495) »
  • Rougier JS  et al. Neuronal precursor cell-expressed developmentally down-regulated 4-1 (NEDD4-1) controls the sorting of newly-synthesized Cav1.2 calcium channels. J Biol Chem : (2011). WB . Read more (PubMed: 21220429) »

See all 4 Publications for this product

Product Wall

Abreviews
Application
ELISA
Sample
Rat Recombinant protein (Rat recombinant protein expressed in E coli)
Specification
Rat recombinant protein expressed in E coli
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Type
Indirect
Username

Abcam user community

Verified customer

Submitted Jun 26 2008

Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (soluble fraction)
Loading amount
25 µg
Specification
soluble fraction
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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