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Overview
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Product nameAnti-CACNB4 antibody [N10/7]
See all CACNB4 primary antibodies -
DescriptionMouse monoclonal [N10/7] to CACNB4
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Host speciesMouse
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SpecificityNo cross reactivity against rat Cav beta 1b, Cav beta 2a and Cav beta 3 in transfected cells.
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IP, Flow Cyt, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to amino acids 458-474 of Rat CACNB4
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Positive control
- Rat brain lysate.
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General notes
The clone number has been updated from S10-7 to N10/7, both clone numbers name the same antibody clone.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberN10/7
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab85788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 58 kDa. | |
| ICC/IF | Use a concentration of 1 - 10 µg/ml. | |
| IHC-Fr | Use a concentration of 0.1 - 1 µg/ml. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 0.1 - 1 µg/ml. |
Target
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FunctionThe beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
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Tissue specificityExpressed predominantly in the cerebellum and kidney.
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Involvement in diseaseGenetic variations in CACNB4 are the cause of susceptibility to idiopathic generalized epilepsy type 9 (IGE9) [MIM:607682]. IGE9 is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Genetic variations in CACNB4 are the cause of susceptibility to juvenile myoclonic epilepsy type 6 (EJM6) [MIM:607682]. EJM6 is a subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similaritiesBelongs to the calcium channel beta subunit family.
Contains 1 SH3 domain. - Information by UniProt
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Database links
- Entrez Gene: 785 Human
- Entrez Gene: 12298 Mouse
- Entrez Gene: 58942 Rat
- Omim: 601949 Human
- SwissProt: O00305 Human
- SwissProt: Q8R0S4 Mouse
- Unigene: 120725 Human
- Unigene: 330223 Mouse
see all -
Alternative names
- CAB4 antibody
- CACB4_HUMAN antibody
- Cacnb4 antibody
see all
Images
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![Flow Cytometry - Anti-CACNB4 antibody [N10/7] (ab85788)](http://a.abcam.com/ps/products/85/ab85788/Images/ab85788-4-ab85788FC.jpg)
Flow Cytometry - Anti-CACNB4 antibody [N10/7] (ab85788)Overlay histogram showing SH-SH5Y cells stained with ab85788 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85788, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-CACNB4 antibody [N10/7] (ab85788)](http://a.abcam.com/ps/products/85/ab85788/Images/CACNB4-Primary-antibodies-ab85788-1.jpg)
Western blot - Anti-CACNB4 antibody [N10/7] (ab85788)All lanes : Anti-CACNB4 antibody [N10/7] (ab85788) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Lysates prepared from CHO-T cells transfected with CACNB4 gene
Predicted band size: 58 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB4 antibody [N10/7] (ab85788)](http://a.abcam.com/ps/products/85/ab85788/Images/CACNB4-Primary-antibodies-ab85788-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB4 antibody [N10/7] (ab85788)ab85788 staining CACNB4 in human hippocapmpus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
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![Western blot - Anti-CACNB4 antibody [N10/7] (ab85788)](http://a.abcam.com/ps/products/85/ab85788/Images/CACNB4-Primary-antibodies-ab85788-5.jpg)
Western blot - Anti-CACNB4 antibody [N10/7] (ab85788)This image is courtesy of an anonymous AbreviewAnti-CACNB4 antibody [N10/7] (ab85788) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
All lanes: Mouse Hippocampal synaptosome tissue lysate (5µg)
Secondary Antibody: An HRP-conjugated Goat Monoclonal 1/10000 dilution
Blocking Step: 5% Milk for 1 hour at 25°C
References
ab85788 has not yet been referenced specifically in any publications.
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