Caco 2 whole cell lysate (ab3950)

Overview

  • Product name
    Caco 2 whole cell lysate
  • General notes
    Cell line: Caco-2 (Human colorectal adenocarcinoma).
    Growth media: Minimum essential medium (Eagle) with 2mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 20% FBS.
  • Tested applications
    Suitable for: WBmore details

Properties

  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Caco-2 cells possess many of the properties of the small intestine. As such, these cells represent a useful and well-accepted tool for metabolism and transport studies (absorption, permeability and/or secretion of drugs/chemicals across the intestinal mucosa).

Applications

Our Abpromise guarantee covers the use of ab3950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Caco-2 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isr ecommended for mini gel.

References

ab3950 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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