Overview

  • Product nameAnti-CAD antibody
    See all CAD primary antibodies
  • Description
    Rabbit polyclonal to CAD
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to a region within residues 1650 - 1700 of Human CAD (NP_004332.2)

  • Positive control
    • Breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, stomach adenocarcinoma, testicular seminoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer will enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Aspartate carbamoyltransferase antibody
      • Aspartate transcarbamylase antibody
      • CAD protein antibody
      • CAD trifunctional protein antibody
      • Carbamoyl phosphate synthetase 2 antibody
      • CPSase ATCase DHOase antibody
      • Dihydroorotase antibody
      • Glutamine dependent carbamoyl phosphate synthase antibody
      • Multifunctional protein CAD antibody
      see all

    Anti-CAD antibody images

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma, using 1/250 ab84474 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human mucinous ovarian carcinoma, using 1/250 ab84474 followed by DAB staining.

    References for Anti-CAD antibody (ab84474)

    ab84474 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84474.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"