Overview

  • Product nameAnti-CAD antibody
    See all CAD primary antibodies
  • Description
    Rabbit polyclonal to CAD
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 1625-1900 of Human CAD (NP_004332).

  • Positive control
    • WB: 293T and H1299 cells IF/ICC: HepG2 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab97340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 243 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Aspartate carbamoyltransferase antibody
    • Aspartate transcarbamylase antibody
    • CAD protein antibody
    • CAD trifunctional protein antibody
    • Carbamoyl phosphate synthetase 2 antibody
    • CPSase ATCase DHOase antibody
    • Dihydroorotase antibody
    • Glutamine dependent carbamoyl phosphate synthase antibody
    • Multifunctional protein CAD antibody
    see all

Anti-CAD antibody images

  • Anti-CAD antibody (ab97340) at 1/1500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 243 kDa
    5% SDS-PAGE.
  • ICC/IF image of ab97340 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CAD antibody (ab97340)

ab97340 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"