Overview

  • Product nameAnti-CAD antibody
    See all CAD primary antibodies
  • Description
    Rabbit polyclonal to CAD
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human CAD (NP_004332.2).

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Aspartate carbamoyltransferase antibody
    • Aspartate transcarbamylase antibody
    • CAD protein antibody
    • CAD trifunctional protein antibody
    • Carbamoyl phosphate synthetase 2 antibody
    • CPSase ATCase DHOase antibody
    • Dihydroorotase antibody
    • Glutamine dependent carbamoyl phosphate synthase antibody
    • Multifunctional protein CAD antibody
    see all

Anti-CAD antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99311 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CAD antibody (ab99311) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 243 kDa


    Exposure time : 10 seconds
  • Detection of CAD by Western Blot of Immunprecipitate.
    ab99311 at 0.1µg/ml staining CAD in HeLa whole cell lysate immunoprecipitated using ab99311 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-CAD antibody (ab99311)

ab99311 has not yet been referenced specifically in any publications.

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