Overview

  • Product name
  • Description
    Rabbit polyclonal to CAD
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1650-1700 of Human CAD (NP_004332.2).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab99312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
    • Cellular localization
      Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Aspartate carbamoyltransferase antibody
      • Aspartate transcarbamylase antibody
      • CAD protein antibody
      • CAD trifunctional protein antibody
      • Carbamoyl phosphate synthetase 2 antibody
      • Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase antibody
      • CPSase ATCase DHOase antibody
      • Dihydroorotase antibody
      • Glutamine dependent carbamoyl phosphate synthase antibody
      • Multifunctional protein CAD antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAD with ab99312 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-CAD antibody (ab99312) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysates at 50 µg
      Lane 2 : HeLa whole cell lysates at 15 µg
      Lane 3 : HeLa whole cell lysates at 5 µg
      Lane 4 : 293T whole cell lysates at 50 µg
      Lane 5 : NIH3T3 whole cell lysates at 50 µg


      Predicted band size : 243 kDa


      Exposure time : 3 seconds

    References

    ab99312 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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