Anti-CAD (phospho T456) antibody (ab111837)

Overview

  • Product nameAnti-CAD (phospho T456) antibody
    See all CAD primary antibodies
  • Description
    Rabbit polyclonal to CAD (phospho T456)
  • Specificityab111837 detects endogenous levels of CAD only when phosphorylated at threonine 456.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from Human CAD around the phosphorylation site of threonine 456 (P-I-TP-P-H).

  • Positive control
    • Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Aspartate carbamoyltransferase antibody
    • Aspartate transcarbamylase antibody
    • CAD protein antibody
    • CAD trifunctional protein antibody
    • Carbamoyl phosphate synthetase 2 antibody
    • CPSase ATCase DHOase antibody
    • Dihydroorotase antibody
    • Glutamine dependent carbamoyl phosphate synthase antibody
    • Multifunctional protein CAD antibody
    see all

Anti-CAD (phospho T456) antibody images

  • ab111837 at 1/50 dilution staining CAD in paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-CAD (phospho T456) antibody (ab111837)

ab111837 has not yet been referenced specifically in any publications.

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