Recombinant Anti-cadherin 10 antibody [EPR5115] (ab134137)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5115] to cadherin 10
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-cadherin 10 antibody [EPR5115]
See all cadherin 10 primary antibodies -
Description
Rabbit monoclonal [EPR5115] to cadherin 10 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human cadherin 10 aa 750-850. The exact sequence is proprietary.
(Peptide available asab185240) -
Positive control
- Human fetal brain, fetal lung and U-87 MG; permeabilized U-87 MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240).
|
Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240). |
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Predominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung. -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1008 Human
- Entrez Gene: 320873 Mouse
- Entrez Gene: 29181 Rat
- Omim: 604555 Human
- SwissProt: Q9Y6N8 Human
- SwissProt: P70408 Mouse
- Unigene: 92489 Human
- Unigene: 117794 Mouse
see all -
Alternative names
- CAD10_HUMAN antibody
- Cadherin 10 (T2 cadherin) antibody
- Cadherin 10 type 2 (T2 cadherin) antibody
see all
Images
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All lanes : Anti-cadherin 10 antibody [EPR5115] (ab134137) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : U-87 MG lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 88 kDa
Observed band size: 115-130 kDa why is the actual band size different from the predicted?The expression profile observed is consistent with what has been described in the literature PMID: 16181616
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Intracellular flow cytometric analysis of permeabilised U-87 MG cells labelling cadherin 10 with ab134137 at 1/10 dilution (red) or rabbit IgG negative (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134137 has been referenced in 1 publication.
- Witte H et al. A Sam68-dependent alternative splicing program shapes postsynaptic protein complexes. Eur J Neurosci 49:1436-1453 (2019). PubMed: 30589479