Anti-cadherin 10 antibody - Aminoterminal end (ab65231)

Overview

  • Product name
    Anti-cadherin 10 antibody - Aminoterminal end
    See all cadherin 10 primary antibodies
  • Description
    Rabbit polyclonal to cadherin 10 - Aminoterminal end
  • Specificity
    This antibody reacts with cadherin 10
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive

  • Positive control
    • CEM cell line lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 95 kDa (predicted molecular weight: 88 kDa).
ELISA 1/1000.

Target

  • Function
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificity
    Predominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Domain
    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD10_HUMAN antibody
    • Cadherin 10 (T2 cadherin) antibody
    • Cadherin 10 type 2 (T2 cadherin) antibody
    • Cadherin 10 type 2 antibody
    • Cadherin-10 antibody
    • Cadherin10 antibody
    • CDH10 antibody
    • T2 cadherin antibody
    • T2-cadherin antibody
    see all

Anti-cadherin 10 antibody - Aminoterminal end images

  • Anti-cadherin 10 antibody - Aminoterminal end (ab65231) at 1/60 dilution + CEM cell line lysates at 35 µg

    Predicted band size : 88 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,72 kDa. We are unsure as to the identity of these extra bands.

References for Anti-cadherin 10 antibody - Aminoterminal end (ab65231)

ab65231 has not yet been referenced specifically in any publications.

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