• Product nameAnti-Cadherin 16 antibody
    See all Cadherin 16 primary antibodies
  • Description
    Rabbit polyclonal to Cadherin 16
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Orangutan
  • Immunogen

    Synthetic peptide: PFLFLEASDR DEPGTANSDL RFHILSQAPA QPSPDMFQLE PRLGALALSP, corresponding to amino acids 144-193 of Human Cadherin 16 (NP_004053).

  • Positive control
    • Human fetal brain lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab80320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.


Anti-Cadherin 16 antibody images

  • Anti-Cadherin 16 antibody (ab80320) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ab80320 staining Cadherin 16 human kidney cells (RPTEC/TERT1) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol, blocked using 5 % BSA, 1 % Triton X-100 for 1 hour at 25°C and then incubated with ab80320 at a 1/100 dilution for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal used at a 1/800 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cadherin 16 with ab80320 at 5µg/ml.

References for Anti-Cadherin 16 antibody (ab80320)

This product has been referenced in:
  • Aschauer L  et al. Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol 33:2535-50 (2013). Read more (PubMed: 23608536) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Cow Tissue lysate - whole (Kidney)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 10 µg
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 07 2016

Thank you for contacting us.

I have added the code and published your review. We are happy that this product worked well for you in this application. Thank you very much for participating in our 100% Abreview testing program.

I hope...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kidney (RPTEC/TERT1))
Specification Kidney (RPTEC/TERT1)
Fixative Methanol
Permeabilization No
Blocking step 5 % BSA, 1 % Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2012