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Synthetic peptide: PFLFLEASDR DEPGTANSDL RFHILSQAPA QPSPDMFQLE PRLGALALSP, corresponding to amino acids 144-193 of Human Cadherin 16 (NP_004053).
Our Abpromise guarantee covers the use of ab80320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cadherin 16 with ab80320 at 5µg/ml.