1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. PB-cadherins may have a role in the morphological organization of pituitary gland and brain tissues.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue labeling Cadherin 22 with ab171616 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-Cadherin 22 antibody (ab171616)
All lanes : Anti-Cadherin 22 antibody (ab171616) at 1/100 dilution
Lane 1 : HepG2 cell line lysate Lane 2 : 293 cell line lysate Lane 3 : K562 cell line lysate