Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Overview

  • Product nameAnti-Cadherin 9 antibody [EPR5113]
    See all Cadherin 9 primary antibodies
  • Description
    Rabbit monoclonal [EPR5113] to Cadherin 9
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the intracellular domain of Human Cadherin 9.

  • Positive control
    • 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
    • Sequence similaritiesContains 5 cadherin domains.
    • DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CADH9_HUMAN antibody
      • Cadherin 9 type 2 antibody
      • Cadherin-9 antibody
      • Cadherin9 antibody
      • CDH 9 antibody
      • CDH9 antibody
      • T1 cadherin antibody
      see all

    Anti-Cadherin 9 antibody [EPR5113] images

    • All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 88 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Cadherin 9 antibody [EPR5113] (ab124803)

    ab124803 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (ES Cells and Neural cells)
    Permeabilization Yes - 0.4%-1% Triton
    Specification ES Cells and Neural cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Dr. Maneeshi Prasad

    Verified customer

    Submitted Jul 21 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"