The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
FunctionInvolved in the cell-cell adhesion. Has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions.
Tissue specificityIsoform 1 is expressed mainly in adult and fetal brain. Isoform 2 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum.
Sequence similaritiesBelongs to the nectin family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe cytoplasmic region mediates interaction with EPB41L1, DLG3, MPP6 and CASK.
Cellular localizationCell junction. Cell membrane. Localized at the cell-cell attached sites of the plasma membrane.