Anti-CAPS2 antibody (ab69794)
Key features and details
- Rabbit polyclonal to CAPS2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAPS2 antibody
See all CAPS2 primary antibodies -
Description
Rabbit polyclonal to CAPS2 -
Host species
Rabbit -
Specificity
specific to CAPS2 and does not recognize CADPS1
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Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CAPS2 (internal sequence).
Database link: Q86UW7 -
Positive control
- Human brain cytoplasmic lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa). |
Target
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Relevance
CADPS2 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family and consists of two members, CAPS1 and CAPS2. Both members play an important role in secretory granule exocytosis. CADPS2 is involved in the release of two neurotrophins, brain-derived neurotrophic factor and neurotrophin-3, from parallel fibers of cerebellar granule cells. CAPS proteins are expressed predominantly in the brain. CADPS2 has been found in various brain regions including the olfactory bulb, cerebrum, hippocampal formation, thalamus, mesencephalic tegmentum, cerebellum, medulla and spinal cord. CADPS2 colocalizes with proteins related to exocytosis (VAMP and SNAP-25) and endocytosis (Dynamin I) in the cell soma and processes of the mesencephalic tegmentum and cerebellum. -
Cellular localization
Cytoplasmic. Membrane-associated to vesicles. -
Database links
- Entrez Gene: 93664 Human
- Omim: 609978 Human
- SwissProt: Q86UW7 Human
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Alternative names
- Cadps2 antibody
- Calcium-dependent activator protein for secretion 2 antibody
- Calcium-dependent secretion activator 2 antibody
see all
Images
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Lane 1 : Anti-CAPS2 antibody (ab69794) at 0.5 µg/ml
Lane 2 : Anti-CAPS2 antibody (ab69794) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 147 kDa
Observed band size: 148 kDa why is the actual band size different from the predicted? -
ab69794 at 5 µg/ml staining CAPS2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Immunofluorescence of CAPS2 in Human Brain cells using ab69794 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab69794 has been referenced in 2 publications.
- Yoo H et al. Down-regulation of habenular calcium-dependent secretion activator 2 induces despair-like behavior. Sci Rep 11:3700 (2021). PubMed: 33580180
- Ayhan F et al. Resolving cellular and molecular diversity along the hippocampal anterior-to-posterior axis in humans. Neuron 109:2091-2105.e6 (2021). PubMed: 34051145